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The changes in the partition coefficient of model proteins (lysozyme, myoglobin, conalbumin, bovine serum albumin) in an aqueous two-phase system formed by polyethylene glycol and dexh-an were examined in order to improve the capacity of counter current distribution (CCD) for the protein fractionation and concentration. The protein distribution pattern in CCD with 30tubes varied with the pH (4.5, 5.5, 6.5, 9.0, 12.0) and KCl concentration (0 mM, 50 mM, 250 mM, 500 mM) of the system. From the mixture of model proteins, pure myoglobin was appeared at the upperphase of 14th tube having 50 mM of KCt at pi-I 5.5 and the upper-phase of 13th tube having 250mM of KCl at pH 6.5. Similarly pure BSA was obtained at the 14th tube having KCl 250mM with pH 4.5, pure lysozyme at the 19th tube having 500mM of KCI at pH 4.5 and the upper-phase of 16th tube 50mM of KCl at pH 5.5.
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